ZP Education Kit - Educate and Inspire

The ZP Education Kit is part of ZP's STEM Mission to Educate and Inspire all generations of scientists.

1. Set Up the Equipment and App:

   • Ensure you have the SenseIt All app set to cyclic voltammetry mode.
   • Name the experiment within the app for easy retrieval later.
   • Confirm that the data will be saved in the cloud (e.g., in "Julie").

2. Prepare the Solution and Electrode:

   • Use 50 µL of 5 mM potassium ferrocyanide solution.
   • Apply the solution onto the screen-printed electrodes.

3. Configure the Settings:

   • Duration: 19 seconds.
   • Initial Voltage: -200 mV.
   • Final Voltage: +600 mV.
   • Scan Rate: 100 mV/s.
   • Cycles: 1.
   • Current Limit: 100 µA.

4. Start the Experiment:

   • Begin the cyclic voltammetry measurement via the app.
   • The experiment will record time versus current during:
     • Oxidation of ferrocyanide to ferricyanide.
     • Reduction of ferricyanide to ferrocyanide.

5. Save and View Results:

   • Confirm that the data has been uploaded to the cloud.
   • Refresh the data view in the app to locate and access the saved results.
   • Review the oxidation and reduction waves for ferrocyanide/ferricyanide.

6. Analyze Data:

   • Use the app to display and interpret the collected data for oxidation and reduction.

With these steps, anyone familiar with the equipment and app should be able to replicate the experiment successfully. Let me know if you need clarification or additional details!

1. Set Up the Equipment and App:

   • Ensure you have the SenseIt All app connected to the potentiostat via Bluetooth.
   • Scan the QR code to change the app mode for conducting multiple experiments in sequence.
   • Name the experiment (e.g., “Test 8”) and save it within a cluster (e.g., “Demo”) for easy data retrieval later.

2. Prepare the Solution and Electrode:

   • Use a HyperValue carbon electrode.
   • Apply 50 µL of 5 mM potassium ferrocyanide solution to the electrode.

3. Experiment Settings:

   • Configure the app to run four experiments in sequence with scan rates of:
     • 50 mV/s (slowest scan).
     • 100 mV/s.
     • 200 mV/s.
     • 300 mV/s (fastest scan).

4. Start the Experiments:

   • Press “Start” in the app to initiate the first scan at 50 mV/s.
   • Subsequent scans will automatically follow at the specified rates (100, 200, and 300 mV/s).

5. Data Upload and Analysis:

   • Once the experiments are complete, confirm the data has been uploaded to the cloud.
   • Access the cloud data via the app and locate your test (e.g., “Test 8”).

6. Visualize and Analyze Results:

   • View cyclic voltammograms for each scan rate (50, 100, 200, and 300 mV/s).
   • Edit the data presentation to enhance clarity or align with desired conventions.
   • Observe the relationship between peak height and the square root of scan rate, as characterized by the Randles-Sevcik equation.

7. Teaching Insights:

   • Reflect on the importance of fast assays in practical applications, emphasizing their relevance in real-world scenarios like healthcare.

This sequence should enable others to repeat the experiment while understanding its teaching objectives. Let me know if you'd like further elaboration!

Here’s a step-by-step guide for repeating Experiment Three from the ZP educational kit:

1. Objective: Understand that voltammetry can be applied in real-world assays, such as analyzing caffeine in Red Bull.

2. Prepare the Sample:

   • Measure 100 µL of Red Bull.
   • Measure 100 µL of ZP’s caffeine buffer (refer to the specific product details).
   • Combine the two solutions in a small vial.

3. Set Up the Equipment:

   • Use the SenseIt All app on your phone.
   • Switch from “Cyclic Voltammetry Mode” to “Caffeine Beverage Mode” by scanning the appropriate QR code.
   • Name the experiment (e.g., “Red Bull”) for data organization in the Julie Cloud.

4. Run the Assay:

   • Pipette 50 µL of the prepared sample onto the sensor.
   • Initiate the assay by pressing “Start” in the app.
   • Monitor the process in real-time as signals are recorded.

5. Data Analysis:

   • Observe the real-time signal, noting interference peaks (e.g., theobromine) and the caffeine peak.
   • Confirm data upload completion to the cloud.

6. Verify Results:

   • Check the caffeine concentration on the Red Bull can (typically 0.3 mg/g).
   • Compare with the experimental result (e.g., 0.328 mg/g).

7. Access Data:

   • Refresh the Julie Cloud interface to locate the experiment file (e.g., “Red Bull”).
   • Review the raw signal, peak identification, and how peak height correlates to caffeine concentration.

This experiment emphasizes the practical applications of voltammetry beyond the lab, particularly for real-world scenarios. Let me know if you need further clarification!